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. 2015 Sep 4;35(19):3312–3323. doi: 10.1128/MCB.00229-15

FIG 4.

FIG 4

ISO induces Nur77 translocation in cardiomyocytes. (A) Myocytes were transduced with either Ad-Nur77 or Ad-DN-Nur77 (MOI, 50). At 24 h after transduction, myocytes were cultured in serum-free medium for 24 h prior to the stimulation with ISO (10 μmol/liter), PE (10 μmol/liter), or ET-1 (100 nmol/liter) for 3 h. Myocytes were then fixed and directly incubated with Cy3-conjugated anti-Flag antibody. Images were visualized using epifluorescence microscope. (B) Myocytes were transduced with either Ad-Nur77 or Ad-DN-Nur77 as indicated above and pretreated with H89 and GFX for 1 h prior to stimulation with ISO (10 μmol/liter), PE (10 μmol/liter), or ET-1 (100 nmol/liter) for 3 h. Myocytes were then fixed and directly incubated with Cy3-conjugated anti-Flag antibody.