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. 2015 Sep 4;35(19):3312–3323. doi: 10.1128/MCB.00229-15

FIG 5.

FIG 5

Knockdown of Nur77 accelerates ISO induced cardiac hypertrophy. (A) Myocytes were transfected with Nur77 siRNA (siNur77) or control siRNA (siCTL). At 72 h after transduction, cells were cultured in serum-free medium for 24 h prior to stimulation with ISO (10 μmol/liter) or PE (10 μmol/liter) for 1 h. The expression of Nur77 was determined by qRT-PCR. The data represent 5 independent experiments. (B) Myocytes were transfected with siNur77) or siCTL. At 72 h after transduction, Nur77 expression was determined by Western blotting after stimulation with ISO (10 µmol/liter) or PE (10 µmol/liter) for 3 h. (C) Myocytes cultured in 24-well plates were transfected with siNur77 or siCTL. At 72 h after transduction, cells were cultured in serum-free medium for 24 h prior to stimulation with ISO (10 μmol/liter) or PE (10 μmol/liter) for 24 h. Leucine incorporation was determined (n = 5). (D) Myocytes cultured in 24-well plates were transfected with siNur77 or siCTL. At 48 h after transduction, myocytes were transfected with ANF-Luc and then stimulated with ISO (10 μmol/liter) or PE (10 μmol/liter) for 24 h. Luciferase assays were performed (n = 5).