Cardiac Nur77 overexpression reduces ISO-induced myocardial hypertrophy. (A) Representative whole-heart picture taken at the end of a 2-week ISO pump infusion in Ad-GFP-PBS (a), Ad-GFP-ISO (b), Ad-Nur77-PBS (c), and Ad-Nur77-ISO (d) groups. (B) Ratio of heart weight (HW) to body weight (BW), *, P < 0.05 versus Ad-GFP-PBS; #, P < 0.05 versus Ad-GFP-ISO. (C) Representative heart tissue sections stained with Masson's trichrome. (D) Representative heart tissue sections stained with Alexa Fluor 594-conjugated wheat germ agglutinin. (E) Summary of cross-sectional area from panel C. *, P < 0.05 versus Ad-GFP-PBS group; #, P < 0.05 versus Ad-GFP-ISO (n = 5 for each group). (F) Expression of ANF, BNP, and β-MHC was examined by qRT-PCR. *, P < 0.05 versus Ad-GFP-PBS group; #, P < 0.05 versus Ad-GFP-ISO (n = 5 for each group).