CRB3A promotes relocalization of phospho-MRLC to cell-cell contacts. F-actin staining (phalloidin; red in the merged picture) and phospho-MRLC immunostaining (P-MRLC; green) in control HeLa cells (A to C), Myc-CRB3A-expressing cells (D to F), Myc-CRB3A-expressing cells transfected with a scrambled siRNA (control RNAi; G to I), Myc-CRB3A-expressing cells knocked down for Ehm2 (Ehm2 RNAi; J to L), Myc-CRB3A-expressing cells knocked down for p114RhoGEF (p114RhoGEF RNAi; M to O), Myc-CRB3A-expressing cells knocked down for RhoA (RhoA RNAi; P to R), or Myc-CRB3A-expressing cells treated with the ROCK1/2 inhibitor Y-27632 (S to U). Scale bar, 20 μm.