Table 3. Primers used in this study.
Primers | Sequence (5′–3′) |
---|---|
tasA-SF | CCCGGATCCACTCTCAAAATACATCAGACAAATAG |
tasA-SR | CGTTCAGGAACGTTCTTGCTTTTTTGCTGTCTAATGTTTC |
tasA-XF | ACAGCAAAAAAGCAAGAACGTTCCTGAACGATAATACATC |
tasA-XR | GGGGTCGACGAATTTTTTCGCATGTTCAAACATT |
tasA-SS | GACTGACGTCATGAGCTGCTGGGTTTTT |
tasA-XX | CCAAGTTCTTTTTCACCGGGAACGCC |
bpr-SF | CCCCGGATCCTAACGCCCTTAAAACGAAATCT |
bpr-SR | TTATTTTTCACATTTCTTTTTCTTTTTCATAGTCTGCCTC |
bpr-XF | ATGAAAAAGAAAAAGAAATGTGAAAAATAACAAGAC |
bpr-XR | CCCCGTCGACTTACTGAACGTCACTCATATC |
bpr-SS | TAGACACGTATTTTCAGCGTGATCC |
bpr-XX | GCTCGGAGGCTATTCAGTTGCGTAT |
epr-SF | CGCGGATCCCCAGGGATGGACAAGAAC |
epr-SR | TAAGCGCTCGTATTCGTTCTCGTTACTGCAGG |
epr-XF | CAGTAACGAGAACGAATACGAGCGCTTATTGG |
epr-XR | AGGCGTCGACAAAGCGGAGGAGAAATACAG |
epr-SS | GCGGGTTTATCCTGTTCTTAATCGG |
epr-XX | GGCACCGTTATTTTCTACAGCCTGG |
mpr-SF | CCCGGATCCACTCTCAAAATACATCAGACAAATAG |
mpr-SR | CGTTCAGGAACGTTCTTGCTTTTTTGCTGTCTAATGTTTC |
mpr-XF | ACAGCAAAAAAGCAAGAACGTTCCTGAACGATAATACATC |
mpr-XR | GGGGTCGACGAATTTTTTCGCATGTTCAAACATT |
mpr-SS | GACTGACGTCATGAGCTGCTGGGTTTTT |
mpr-XX | CCAAGTTCTTTTTCACCGGGAACGCC |
vpr-SF | CCCCGGATCCTAACGCCCTTAAAACGAAATCT |
vpr-SR | TTATTTTTCACATTTCTTTTTCTTTTTCATAGTCTGCCTC |
vpr-XF | ATGAAAAAGAAAAAGAAATGTGAAAAATAACAAGAC |
vpr-XR | CCCCGTCGACTTACTGAACGTCACTCATATC |
vpr-SS | TAGACACGTATTTTCAGCGTGATCC |
vpr-XX | GCTCGGAGGCTATTCAGTTGCGTAT |
nprE-SF | CCCCGGATCCTAACGCCCTTAAAACGAAATCT |
nprE-SR | TTATTTTTCACATTTCTTTTTCTTTTTCATAGTCTGCCTC |
nprE-XF | ATGAAAAAGAAAAAGAAATGTGAAAAATAACAAGAC |
nprE-XR | CCCCGTCGACTTACTGAACGTCACTCATATC |
nprE-SS | TAGACACGTATTTTCAGCGTGATCC |
nprE-XX | GCTCGGAGGCTATTCAGTTGCGTAT |
aprE-SF | CCCCGGATCCTAACGCCCTTAAAACGAAATCT |
aprE-SR | TTATTTTTCACATTTCTTTTTCTTTTTCATAGTCTGCCTC |
aprE-XF | ATGAAAAAGAAAAAGAAATGTGAAAAATAACAAGAC |
aprE-XR | CCCCGTCGACTTACTGAACGTCACTCATATC |
aprE-SS | TAGACACGTATTTTCAGCGTGATCC |
aprE-XX | GCTCGGAGGCTATTCAGTTGCGTAT |
The restriction enzyme cleavage sites are underlined.