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. 2015 Sep 8;5:13613. doi: 10.1038/srep13613

Figure 5. K+ inhibitors blocked amebic cytotoxicity and K+ activation by E. histolytica in intestinal epithelial cells and macrophages.

Figure 5

(a) Chemical inhibition of ion transport blocked amebic cytotoxicity. Cells were switched to the indicated concentrations of chemicals immediately prior to the addition of E. histolytica. (b) Specific K+ channel inhibitors blocked amebic cytotoxicity. Cells were treated with inhibitors for 30 minutes prior to the addition of E. histolytica. (c) K+ channel activation by E. histolytica. Fluorescence values correspond to thallium influx through open K+ channels. F values were normalized to the initial baseline value. E. histolytica (+EH) or vehicle (−EH) was added after 40 seconds. The mean of 3 biological replicates and the range is shown (+EH), −EH values are single measurements. (d) Inhibitors blocked K+ channel activation by E. histolytica. The mean of the area under the curve (AUC) for inhibitor-treated cells (KCl and ChoCl: 25 mM, 293B, CLO, PAX: 10 μ0) with (+EH) and without E. histolytica (−EH) is shown. The AUC (% of control) for each inhibitor was normalized to the media control (+EH was normalized to +EH control, −EH was normalized to −EH control). (+EH is the AUC of three biological replicates; error bars represent the range of measurements. *P < 0.001 calculated for each inhibitor relative to untreated cells by Fisher’s LSD test.