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. 2015 Sep 8;5:13824. doi: 10.1038/srep13824

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Platelets were preincubated with or without CA in the presence of CaCl₂ (1 mM) at 37 °C for 20 min and then stimulated with ADP for 20 min. cAMP level was determined with a Cyclic AMP XP® Chemiluminescent Assay Kit. CA significantly increased cAMP levels in ADP-stimulated platelets in a concentration-dependent manner. Data are expressed as means ± SEM (n = 10). *p < 0.05 vs control group, ^#p < 0.05 vs ADP group.