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. 2015 Sep 8;5:13824. doi: 10.1038/srep13824

Figure 9. CA attenuates ADP-activated platelet p38MAPK, ERK and JNK phosphorylation.

Figure 9

Washed platelets were incubated with CA or vehicle for 20 min and then stimulated with ADP for 20 min. Platelet proteins were then extracted and specific antibodies were used to measure the levels of total and phosphorylated p38 (A), ERK (B), JNK (C), and Akt (D). CA attenuated the phosphorylation of p38, ERK and JNK but not of Akt (Ser473) in the activated platelets. Images are representative of three separate experiments. The relative protein expression levels were quantified by Quantity One software. Data represent the mean ± SEM of at least three independent experiments performed in triplicate. *p < 0.05 vs control group; #p < 0.05 vs ADP group.