Fig. 2.

Kinetics of the immunoblot reaction against B. besnoiti tachyzoite antigens in each rabbit group. The immunoblot pattern is presented for one rabbit per group. Rabbits were experimentally subcutaneously challenged with 66 μg of ovalbumin (group C, a), 6.10⁶ tachyzoites (group T, b) and 6.10⁶ bradyzoites (group B, c) of B. besnoiti at day 0. Control sera (−) were obtained from rabbits inoculated subcutaneously with 66 μg of ovalbumin. Positive sera (+) were obtained from one rabbit inoculated subcutaneously with 6.10⁶ bradyzoites of B. besnoiti. Ten tachyzoite antigens recognized by B. besnoiti naturally infected cows are indicated by arrowheads