Table 1.
The number of seropositive conversion in each group of rabbits from pre-challenge (day –4) to euthanasia (day 70) determined by Western blot with non-reduced B. besnoiti tachyzoite antigens
Day | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Group | –4 | 2 | 7 | 9 | 14 | 17 | 21 | 28 | 35 | 42 | 49 | 56 | 63 | 70 |
Control (n = 6) | 0/6 | 0/6 | 0/6 | n.d. | 0/6 | n.d. | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Tachyzoites (n = 5) | 0/5 | 0/5 | 0/5 | n.d. | 4/5 | n.d. | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 |
Bradyzoites (n = 5) | 0/5 | 0/5 | 0/5 | 0/5 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 |
Rabbits were experimentally subcutaneously inoculated at day 0 with 66 μg of ovalbumin (control group), 6.106 tachyzoites (tachyzoite group) and 6.106 bradyzoites (bradyzoite group)
n.d. not done