Table 4.
Quantitative PCR Ct values of B. besnoiti in tissues of inoculated rabbits at euthanasia
| Tachyzoite-infected rabbits | Bradyzoite-infected rabbits | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tissue location | T01 | T02 | T01 | T02 | T01 | B01 | B02 | B04 | B05 | B06 |
| Nasal mucosa | – | – | – | – | – | 24.6 | 25.5 | 31.2 | 29.2 | 35.9 |
| Tracheal mucosa | – | – | – | – | – | – | 37 | 34.6 | 37.7 | – |
| Lung | – | – | – | – | – | 32.2 | 36.7 | 35.2 | 35.9 | – |
| Heart | – | – | – | – | – | 35.6 | – | – | – | – |
| Pancreas | – | – | – | – | – | 38 | – | – | – | – |
| Liver | – | – | – | – | – | – | – | – | – | – |
| Gallbladder | – | – | – | – | – | – | – | – | – | – |
| Kidney | – | – | – | – | – | – | – | – | – | – |
| Spleen | – | – | – | – | – | – | – | – | – | – |
| Diaphragm | – | – | – | – | – | – | – | – | – | – |
| Right eye | – | – | – | – | – | 32 | 36.7 | – | 35.2 | – |
| Ovary or testis | – | – | – | – | – | – | 35 (ovary) | – | 38 (testis) | – |
| Vagina or penis | – | – | – | – | – | 27.9 (penis) | – | – | 27.6 (penis) | – |
| Scrotum/vulvar mucosa | – | – | – | – | – | – | – | – | 38 | – |
Ct values are reported in the table at day 70. Rabbits were experimentally subcutaneously infected with either 6.106 B. besnoiti tachyzoites (tachyzoite group) or bradyzoites (bradyzoite group) at day 0
–: no Ct