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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: Neuron. 2015 Jul 15;87(2):424–436. doi: 10.1016/j.neuron.2015.06.030

Figure 1. Characterization of PV-Cre knock-in mouse and two-photon in vivo imaging of PV+ interneurons.

Figure 1

(A) Representative PV-Cre;Ai14 mouse cortex stained for parvalbumin (PV) with V1 delineated. (B–D) Maximal projections showing co-localization of PV and tdTomato in cortical layers 2–4. In these panels all PV+ neurons are tdTomato+. Arrows indicate tdTomato+/PV− neurons. (E) Summary data on PV-tdTomato co-localization in V1. Counts obtained from cortical layers 2–5. (F) Dichoptic stimulus presentation used to evoke calcium responses. OGB-1 bulk loaded in mouse V1 binocular zone. (G) Two-photon image of OGB-1 In vivo. (H) Same as in (G) for tdTomato. (I) Merged OGB-1 and tdTomato signals. Cells with co-localized fluorescence (putative PV+ interneurons) appear yellow (arrows).