Table 1.
Loading MOI |
2 × 107 reo | PBS | |||
---|---|---|---|---|---|
0 | 0.1 | 10 | |||
T cells | 0 | 5000 | 0 | 0 | 0 |
0 | 10 000 | 0 | 0 | 0 | |
0 | 100 | 0 | 0 | ||
iDC | 0 | 0 | 0 | ||
0 | 0 | 0 | |||
0 | 0 | 0 | |||
mDC | 0 | 300 000 | 400 | ||
0 | 4000 | 0 | |||
0 | 100 000 | 8000 |
Mice (3 per group) were immunized with 108 p.f.u. reovirus i.p. After 2 weeks the mice were seeded s.c. with 2 × 105 B16tk cells. After a further 10 days, the mice were treated with PBS, 2 × 107 pfu reovirus or 2 × 106 T cells, iDC or mDC loaded with reovirus as indicated. After 3 days TDLN were removed and viral delivery was determined by plaque assay.