Table 2.
Early tumour burden in reovirus-immune mice after treatment
| Loading MOI |
2 × 107 reo | PBS | |||
|---|---|---|---|---|---|
| 0 | 0.1 | 10 | |||
| T cells | >30 | 7 | >30 | >30 | >30 |
| >30 | 0 | >30 | >30 | >30 | |
| >30 | 15 | >30 | >30 | ||
| iDC | >30 | >30 | >30 | ||
| >30 | >30 | >30 | |||
| >30 | >30 | >30 | |||
| mDC | >30 | 0 | 15 | ||
| >30 | 70 | 25 | |||
| >30 | 0 | 0 | |||
Mice (3 per group) were immunized with 108 pfu reovirus i.p. After 2 weeks the mice were seeded s.c. with 2 × 105 B16tk cells. After a further 10 days, the mice were treated with PBS, 2 × 107 p.f.u. reovirus or 2 × 106 T cells, iDC or mDC loaded with reovirus as indicated. After 3 days TDLN were removed and metastatic burden was determined by puromycin-resistant colony outgrowth.