| Reagent | Volume (μL) |
|---|---|
| Cleaned DSN treated reaction mix | 30 |
| 5X Phusion buffer | 10 |
| Phusion polymerase | 0.5 |
| 25 mM dNTP | 0.5 |
| PCR Primer PE 1.0 | 0.5 |
| PCR Primer PE 2.0 | 0.5 |
| Nuclease-free water | 8 |
| Total Volume Per Sample | 50 |
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