Table 2.

PCR results obtained according to the DNA extraction method and the Mycoplasma agalactiae concentration used

M. agalactiae concentration (CFU/ml)	Method of extraction
	Tola et al. (1997) [8]			Commercial kitc
	Negative	Positive	Se (%), 95 % CI	Negative	Positive	Se (%), 95 % CI
101	30	0	0	30	0	0
102	30	0	0	29	1	3.3, (−3.1 %, 9.8 %)
103	30	0	0	24	6	20, (5.7 %, 34.3 %)
104	28	2	6,7, (−2.3 %, 15.6 %)	22	8	26.7, (10.8 %, 42.5 %)
105	15	15	50, (32.1 %, 67.9 %)	8	22	73.3, (57.5 %, 89.2 %)
106	2	28	93,3, (84.4 %, 102.3 %)	0	30	100
107	0	24	100	0	24	100
Total	135	69	33.8,a (37.8 %, 51.4 %)	113	91	44.6,b (27.3 %, 40.3 %)

Se sensitivity at each concentration

^{a, b}: Values with different superscripts differ significantly (p < 0.05)

^c: High Pure PCR Template Preparation Kit, Roche Diagnostics