Figure 2. Kit gene expression and location.

(A) Kit protein was detected on Western blotting of the cerebrum and cerebellum. The signals of Kit in WS mice were weaker than in wild-type. (B) FISH to metaphase chromosome spreads of WS mice. FITC (green) signals, which reflected the Fgf5 gene locus, were observed on both copies of chromosome 5. In contrast, Cy3 (red) signals, reflecting the Kit gene locus, were only seen on one copy (white arrow).