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. 2015 Sep 8;5:13632. doi: 10.1038/srep13632

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(A) Southern blotting of Exoc1^−/+ and Exoc1^+/+ mice. MfeI-digested DNA fragments were detected by 5′ outer and LacZ inner probes, and the BamHI-digested DNA fragments were detected by 3′ outer probes. (B) Western blotting showed that Exoc1 protein expression was reduced in Exoc1^−/+ mice. (C–E) Cultivated blastocysts from Exoc1^−/+ intercrosses. TG-like cells with an enlarged nucleus (arrowheads) were seen in cultivated embryos of either genotype. Dome-shaped colonies (arrows) were not seen in Exoc1^−/−. M, MfeI; Ba, BamHI; Scale bar = 100 μm.