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. 2015 Sep 7;6(1):162. doi: 10.1186/s13287-015-0156-4

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© Hata et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Differential potential and proliferative capacity of freshly isolated dental pulp stem cells (fresh-DPSCs) and cryopreserved dental pulp stem cells (cryo-DPSCs). a Adipogenic differentiation was observed by oil red O staining. Osteogenic differentiation was observed by alkaline phosphatase (ALP) activity. Bar = 250 μm. b The growth of fresh-DPSCs and cryo-DPSCs was measured as the cell number counted with a hemocytometer on day 3 and day 5 after seeding. c The proliferative activity of fresh-DPSCs and cryo-DPSCs was determined by the MTT assay on day 3 and day 5. Results are expressed as means ± SEM (n = 9)