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. 2015 Sep 8;5:13778. doi: 10.1038/srep13778

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The 2-DE gel for E-3 latex was stained with Pro-Q Diamond dye to detect phosphoproteins (a, red). It was then restained with SYPRO Ruby (b, green). The same gel was restained by G250 (c, blue). The combination image of Pro-Q Diamond and SYPRO Ruby are presented to demonstrate the specific phosphorylated proteins (d, merged). The latex proteins obtained from the D-0 (e-g) and D-3 (h-j) plants were also stained by Pro-Q Diamond and SYPRO Ruby. Finally, 59 phosphoproteins (P1-P59) were identified by MS (d).