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. 2015 Sep 1;3:e1213. doi: 10.7717/peerj.1213

Figure 2. The expression W01A8.1::gfp reporter genes in transgenic strains.

Figure 2

W01A8.1a/c::GFP is shown in (A, C, E, G), and (I), and corresponding areas in Nomarski optics are shown in (B, D, F, H) and (J). (A) The onset of expression of W01A8.1a/c::GFP in epidermal cells and in intestinal cells of three-fold embryo. (C) The expression of W01A8.1a/c::GFP in intestinal cells of an L2 larva. (E) and (G) W01A8.1a/c::GFP expression in epidermal cells and intestinal cells of a young adult hermaphrodite. (G) GFP fluorescence around lipid droplet-like structures in the intestine that are marked by arrows and arrowheads. Corresponding image in Nomarski optics is in (H). (I) A higher magnification the lipid droplet-like structures in epidermal cells labeled by W01A8.1a/c::GFP (shown in Nomarski optics in the J). (K) Lipid droplets of an unfixed intestine labeled by W01A8.1b::GFP (corresponding Nomarski image is in L). (M, N) and (O) Part of the intestine of an adult larva expressing W01A8.1b::GFP (M) with corresponding staining of lipid droplets by LipidTox (N). (O) LipidTox-positive lipid droplets (red) with W01A8.1b::GFP on the periphery (green) in this merged view. Bars represent 50 µm in (B, H, J, L) and (N) and 100 µm in (D) and (F).