Skip to main content
. 2015 Sep 1;3:e1213. doi: 10.7717/peerj.1213

Figure 3. Expression of human perilipins fused to GFP in C. elegans.

Figure 3

(A–C) Expression of human PLIN1::GFP in live transgenic C. elegans. PLIN1::GFP is localized on vesicles with an appearance of lipid droplets. PLIN2::GFP (D–F) is localized in transgenic animals on vesicular structures with an appearance of lipid droplets similarly as PLIN1::GFP. PLIN3::GFP (G, H and I) yields a more diffuse cytoplasmic pattern with faintly stained vesicular structures. (A, D) and (G) and details in (C, F) and (I) show GFP in fluorescence microscopy and (B, E) and (H) corresponding areas to (A, D) and (G) in Nomarski optics. (J, K) and (L) show PLIN2::GFP in fluorescence microscopy (J) in fixed C. elegans stained with LipidTox (K). The area indicated by the white rectangle in (J) and (K) is magnified and merged for co-localization of PLIN2::GFP (green) and LipidTox (red) in (L). Arrows indicate lipid droplets clearly marked by GFP with the LipidTox positive content. Bars represent 50 µm.