Figure 7.

Proliferation of microglial and BMDM/DC populations during EAE. Representative flow cytometry profiles of six individual central nervous system suspensions prepared from mice with severe EAE showing BrdU incorporation by CD11c⁻ microglia (green) and CD11c⁺ microglia (red) based on negative control (blue) (A). Flow cytometry analysis shows a significant increase in percentage of proliferating (incorporating BrdU) microglia during EAE compared to naive controls (B). Expression of CSF1R in fluorescence-activated cell sorted myeloid cells (CD11c⁺ microglia, CD11c⁻ microglia, and CD45^highCD11c⁺) from the central nervous system was analyzed by quantitative real-time PCR (C). Data are presented as means ± SEM of three individual experiments (n ≥ 5, where n represents a pool of 2–3 individual mice) *P < 0.05.