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. 2015 Aug 26;6:663. doi: 10.3389/fpls.2015.00663

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Copyright © 2015 McCormack, Liu, Jordan and Pajerowska-Mukhtar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Unfolded protein response (UPR) target genes are upregulated during Tm-induced ER stress. Five-day-old Arabidopsis seedlings were pre-grown in liquid MS medium, then subjected to stress by replacing the media with liquid MS supplemented with designated concentrations of Tm for 3 days. The recovery was performed by incubating stressed seedlings in liquid MS medium for another 3 days. The transcript accumulation of (A) CRT1, (B) CRT3, (C) CNX1, and (D) bZIP60 splicing were measured by real-time PCR and normalized to a housekeeping gene UBQ5. Statistical analysis was performed with Student’s t-test comparing values from Tm-exposed seedlings to regular MS-grown seedlings, *p < 0.05, **p < 0.01, ***p < 0.001. Experiments were performed in at least three independent biological replications. Error bars represent SE between these replications.