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. Author manuscript; available in PMC: 2015 Sep 8.
Published in final edited form as: Mol Oral Microbiol. 2012 Dec 17;28(3):181–191. doi: 10.1111/omi.12013

Figure 4.

Figure 4

Protease expression and activity in T. denticola strains. Panel A: Immunoblots of T. denticola strains probed with antibodies specific for components of the dentilisin complex. Blots were probed with polyclonal antibodies specific for the proteins indicated to the right of each blot. All antibodies were raised against T. denticola 35405 proteins expressed in E. coli. Strain names are listed above each lane. Approximate sizes in kDa of the reactive bands are indicated to the left of each blot. Panel B: PrtP protease activity in 5-day T. denticola cultures. Protease activity of individual strains, assayed by change in absorbance at 405 nm, is expressed as a percentage relative to the SAAPFNA activity of strain 35405. Error bars represent the standard error of four independent experiments including triplicate samples.