Table 2. Oligonucleotide primers used in this study.
| Primer | Sequencea | Notesb |
|---|---|---|
| CX816 | CGGGACTTCCATGGCAACCCCATTTG | mid-prtP w/NcoI [F] |
| CX822 | GCTCGAGCGGATATTCTACATAGAACCAATC | prtP 3′ end w/XhoI [R] |
| CX834 | GATAACTGCAGATCAAATGAAGGCTCTGC | mid-prtP w/ PstI P1 [F] |
| CX847 | cttcttaattacTCAGTGGTGGTGGTGGT | prtP6xHis-ermB P2 [R] |
| CX848 | CACCACTGAgtaattaagaaggagtgattac | prtP6xHis-ermB [F] |
| CX849 | ATATTAACGcctaataatttatctacattcc | ermB-TERMprtP [R] |
| CX850 | gataaattattaggCGTTAATATGGGTAATTAGG | ermB-TERMprtP [F] |
| CX819 | CTTCAATGGGAAGAAGGAAG | past prtP-->tuf [R] |
| CX859 | GATCAAATGAAGGCTCTGC | mid-prtP [F] |
| CX636 | GCGCCGCCCTAATTAC | past prtP [R] |
| CX637 | GTTTGGTACGGTCGAAAC | prtP 1188 [F] |
a
Engineered restriction enzyme sites underlined; Upper case: prtP homology; Lower case: ermB homology.
b
Target; orientation with respect to prtP: forward [F]; reverse [R]).