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. 2015 Oct 15;414:120–131. doi: 10.1016/j.mce.2015.07.025

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 7 — Effects of embryonic glucocorticoid receptor manipulation on adult heart gene expression mRNA expression in adult isolated hearts after glucocorticoid receptor (GR) manipulation with either GR agonist dexamethasone (Dex) [100 μM], or targeted GR knockdown using morpholino (GR Mo). Genes of interest were (A) Glucocorticoid receptor (gr) (B) Insulin like growth factor (igf), (C) ventricular myosin heavy chain (vmhc), (D) phospholamban (plb), (E) Ryanodine receptor (ryr), (F) sarco-endoplasmic reticulum Ca2+ ATPase (serca), (G) myocyte enhancer factor 2 c (mef2c), (H) GATA transcription factor 4 (gata4) and (I) Homeobox protein NKX2.5 (nkx2.5). Relative mRNA expression was determined from isolated hearts at 120 dpf, mean of n = 3 (5 hearts per n) ± SEM, all data analysed compared to control by one-way ANOVA and Dunnett's post hoc test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001).