Figure 6. Characterization of VGLUT3+ Neurons and Primary Afferent Input.
(A) Lsl-tdTom;VGLUT3Cre neurons were recorded using transverse spinal cord slices with the dorsal root and DRG still attached. Cells were filled with Alexa-488 and biotin for post-hoc reconstruction. Scale bars = 200 μm (upper left) and 50 μm.
(B) Example traces of tonic, phasic and single firing patterns recorded in tomato+ neurons.
(C) The tomato+ neurons receive monosynaptic or polysynaptic Aβ inputs.
(D) Incidence of firing patterns and afferent inputs of tomato+ neurons.
(E) Morphology of lamina III tomato+ neurons. Scale bar = 50 μm.