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Oligomeric state of HsAPC-1 RD under different conditions.
| Original oligomeric state | Conditions of second SEC column |
Elution volume (mL) | Estimated molecular weight (kDa) | Oligomeric state after second SEC column | ||
|---|---|---|---|---|---|---|
| Ca2 + | EGTA | DTT | ||||
| Monomer | ✓ | 93.8 | 25 | Monomer | ||
| Dimer | ✓ | 84.8 | 51 | Dimer | ||
| Dimer | ✓ | ✓ | 93.8 | 25 | Monomer | |
| Monomer | ✓ | 90.3 | 34 | Monomer | ||
| Dimer | ✓ | 79.3 | 80 | Dimer | ||
| Dimer | ✓ | ✓ | 90.3 | 34 | Monomer | |
