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. 2015 Sep 7;15:315. doi: 10.1186/s12906-015-0839-5

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© Jiang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The content of cNOS, tNOS, iNOS and NO in cell supernatants. The content of cNOS, tNOS, iNOS and NO in cell supernatants was determined by nitrate reductase method. a The content of cNOS in the supernatant was assayed after HUVECs were incubated with calycosin in different concentration (0 μg/ml, 0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml or 20 μg/ml) for 24 h; b The content of cNOS in the supernatant was assayed after HUVECs were incubated with 10 μg/ml calycosin for different time (0 h, 4 h, 8 h, 12 h, 24 h or 48 h); c The content of cNOS, tNOS and iNOS in cell supernatants; d The content of NO in cell supernatants. ^△ P < 0.05, compared with normal HUVECs; ^★ P < 0.05, compared with LPS-induced inflammatory and injured HUVECs