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. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: Biochem J. 2014 Dec 15;464(3):377–386. doi: 10.1042/BJ20141164

Figure 5. POLH 3′UTR is responsive to PCBP1.

Figure 5

(A) Schematic presentation of mutant p53 (R175H) reporter vectors that carry none, the full-length, or a part of POLH 3′UTR. Ctrl: no POLH 3′UTR; 3′UTR: the full-length POLH 3′UTR from nt 2447–8412; A: nt 2447–3607 from POLH mRNA; B: nt 3515–4619 from POLH mRNA; C: nt 4580–5879 from POLH mRNA; D: nt 5843–7177 from POLH mRNA; E: 7032–8412 from POLH mRNA. (B) The reporter vectors were transfected into MIA-PaCa2 cells transduced with a lentivirus expressing luciferase shRNA or PCBP1 shRNA (shPCBP1) for 3 days. Western blots were performed to determine the levels of the reporter (HA-tagged mutant p53 R175H), endogenous POLH, PCBP1, and actin. Western blots shown in the figure were representative of three independent experiments. The values below the strips were the relative intensities normalized to actin.