Table 1. Brain regions and stains.
For “A” score^ | For “B” score# | For “C” score* | |
---|---|---|---|
Aβ Stain | NFT Stain | NP Stain | |
Middle frontal gyrus | X | X | X |
Superior and middle temporal gyri | X | X | X |
Inferior parietal lobule | X | X | X |
Occipital Cortex (BA 17 & 18) | X | ||
Hippocampus and entorhinal cortex | X | X | |
Basal ganglia at anterior commissure | X | ||
Midbrain including substantia nigra | X | ||
Cerebellar cortex | X |
Staining methods used by different sites included:
immunohistochemistry for Aβ (antibodies (source) 6E10 (Covance), 6F/3D (DAKO), 4G8 (Covance), NAB228 (University of Pennsylvania), 10D5 (Elan), and AB5074P and AB5078P (Chemicon);
immunohistochemistry for neurofibrillary degeneration (antibodies PHF-1 (Dr. Peter Davies), Tau (DAKO), AT8 (Autogen Bioclear), MN1020 (Thermo Scientific), and
histochemistry (Thioflavin S or Bielschowsky stain) or immunohistochemistry (NM1020 or PHF-1) for neuritic plaques. Staining methods used by site are shown in Table S1.