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. Author manuscript; available in PMC: 2016 Jan 20.
Published in final edited form as: Immunity. 2014 Dec 25;42(1):108–122. doi: 10.1016/j.immuni.2014.12.018

Figure 6. T-bet Quantitatively Controls IFN-γ Expression in Fully Differentiated Th1 Cells.

Figure 6

(A) Experimental setup. Th1 cells were sorted by secreted IFN-γ amounts and transduced with a T-bet-encoding or control retrovirus. IFN-γ expression was analyzed in transduced (hCD4+) cells 2 days later.

(B) Frequencies of IFN-γ+ cells and geometric mean of IFN-γ in IFN-γ+ cells (bold numbers).

(C) Relative increase in IFN-γ expression probability and per-cell amount upon T-bet overexpression (means + SD).

(D and E) Unsorted Th1 cells were transduced with a T-bet-encoding retrovirus and analyzed 2 days later.

(D) Counterstaining of T-bet and hCD4.

(E) Frequencies of IFN-γ+ cells and geometric mean of IFN-γ in IFN-γ+ cells (bold numbers) in cells overexpressing different amounts of hCD4, i.e., T-bet.

Representative results of (B, D, E) or pooled data from (C) two independent experiments are shown.