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. Author manuscript; available in PMC: 2015 Sep 8.
Published in final edited form as: Prostaglandins Leukot Essent Fatty Acids. 2015 Apr 11;97:27–34. doi: 10.1016/j.plefa.2015.03.002

Fig. 4.

Fig. 4

Effect of NS-398 on (A) PGE2 secretion, (B) TNFα, and (C) IL-6 gene expression. RAW 264.7 cells were pretreated with NS-398 (10 μM, 18 h) and then stimulated with ultra-pure LPS (100 ng/mL). (A) PGE2 in culture supernatant was determined by ELISA after 6 h of ultra-pure LPS stimulation. **P <0.01 vs. control determined by unpaired Student t test. (B) TNFα and (C) IL-6 gene expression were determined after 3 or 6 h of ultra-pure LPS stimulation. Values are mean±SD of three independent experiments. *P<0.05 determined by two-way repeated measures ANOVA adjusted by Sidak’s test for multiple comparisons. Values are mean±SD of three independent experiments.