Figure 4.

Glomerular area and mesangial expansion. Glomerular area (A) and mesangial expansion (B) were increased in diabetic mice treated with vehicle. Treatment with Ang-(1–7) significantly reduced both of these parameters. Addition of A-779, PD123319 and L-NAME, but not losartan or icatibant, resulted in inhibition of the effects of Ang-(1–7) (Hz – heterozygous; db – diabetic). **P < 0.01; ***P < 0.001; ****P < 0.0001. Significantly increased compared with db + Ang-(1–7), †P < 0.05; ††P < 0.01; or ††††P < 0.0001; calculated using one-way anova.