Figure 5.

Electrical resistance of murine endothelial cell monolayers treated with either vehicle or (A) PGE₂ or the (B) EP₄ agonist ONO AE1-329 at the indicated concentrations. (C). Cells were treated with PGE₂ (30 nmol·L⁻¹) or (D) the EP₄ agonist ONO AE1-329 (30 nmol·L⁻¹) alone or in the presence of the EP₄ antagonist ONO AE3-208 (1 μmol·L⁻¹, 15 min pretreatment). Data are shown as mean normalized resistance + SEM of four independent experiments performed in duplicate. Data were analysed by two-way anova for repeated measurements and multiple comparisons were calculated with Tukey’s post test. ***P < 0.001 as compared with vehicle; ###P < 0.001 as compared with PGE₂ or the EP₄ agonist. (E) EP₄ receptor expression on murine pulmonary microvascular cells using flow cytometry (typical example of n = 3). AB, antibody.