Figure 6.

Electrical resistance of human endothelial cell monolayers treated with vehicle or LPS (1 μg·mL⁻¹) in the absence or presence of (A,C) PGE₂ (30 nmol·L⁻¹), (B,D) the EP₄ receptor agonist ONO AE1-329 (100 nmol·L⁻¹). (C,D) Cells were pretreated with the EP₄ receptor antagonist ONO AE3-208 (300 nmol·L⁻¹) for 15 min. Data are shown as mean normalized resistance + SEM of 4–7 independent experiments performed in duplicate. Data were analysed by two-way anova for repeated measurements and multiple comparisons were calculated with Tukey’s post test. ***P < 0.001 as compared with vehicle; ###P < 0.001 as compared with LPS.