TH was localized in beta cells, whereas DAT was not confined to α-cells, beta cells or δ-cells. IHC analyses of pancreatic islets from HFD-fed mice. Paraffin-embedded sections were co-stained with anti-TH (red) and anti-insulin (green) (A), anti-DAT (red) and anti-insulin (green) (B), or anti-DAT (red) and anti-glucagon (green) (C) antibodies. Scale bar, 5 μm; magnification, ×600. (D) Serial paraffin-embedded sections were stained with anti-DAT and anti-somatostatin antibodies. Scale bar, 20 μm; magnification, ×400.