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. 2014 Sep;99(9):1465–1471. doi: 10.3324/haematol.2013.100917

Figure 1.

Figure 1.

Functional co-operation of NUP98-NSD1 and FLT3-ITD in vitro. (A) Schematic outline of the experimental strategy to address the functional co-operation between NUP98-NSD1 and FLT3-ITD. (B) Serial plating in methylcellulose of cells expressing NUP98-NSD1, FLT3-ITD or both, of Balb/C (upper panel) and C57BL/6 mice (lower panel): NUP98-NSD1 increased colony formation of Balb/C-but not of C57BL/6-derived cells, whereas co-expression of FLT3-ITD supported colony formation in both strains. No significant re-plating activity was observed upon expression of FLT3-ITD or the control vector (GFP) in cells of both strains. (C) Proliferation of cells expressing NUP98-NSD1, FLT3-ITD or both in liquid culture. Co-expression of NUP98-NSD1 and FLT3-ITD significantly increased proliferation compared to single transduced cells. Proliferation was assessed in triplicates using trypan blue staining. Error bars represent the standard deviations from 5 independent experiments with Balb/C and 3 independent experiments with C57BL/6-derived cells (t-test, two-tailed, unpaired/unequal variances, *P ≤ 0.05, **P≤0.01).