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. 2015 Aug 11;3(8):e12481. doi: 10.14814/phy2.12481

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Perilipin (PLIN)4 is close to the sarcolemma of skeletal muscle fibers whereas PLIN5 is spread uniformly. (A) Representative images (630X) from confocal fluorescence microscopy of semithin (5 μm) cross sections of paraffin-embedded human m. vastus lateralis biopsy (n = 10). Dystrophin (red, rabbit anti-dystrophin) binds to the outside of the sarcolemma, whereas PLIN4 (green, guinea pig anti-PLIN4) is located at or just beneath the sarcolemma. (B) The image (400X) was obtained by confocal fluorescence microscopy of sections of paraffin-embedded biopsies from m. vastus lateralis (n = 10), stained with antibodies against PLIN4 (red, rabbit anti-PLIN4) and PLIN5 (green, guinea pig anti-PLIN5).