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. 2015 Aug 11;3(8):e12481. doi: 10.14814/phy2.12481

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Perilipin (PLIN)4 is associated with the membrane. (A) PLIN4 protein was analyzed by western blotting in different fractions (membrane/organelles, cytoskeleton, cytosol, and nuclear) from human muscle biopsies (n = 3). Each lane was loaded with 8 μg protein and in the membrane/organelles fraction a band was observed ∼ 120 kDa using a PLIN4-specific antibody (arrow). (B) It shows correlations between change in PLIN4 mRNA expression and change in liquid droplets (LDs) in different subcellular locations after 12 weeks of training. mRNA expression was determined with high throughput sequencing expressed as fragments per Kilobase per million fragments mapped (FPKM). The area of LDs was calculated from LD diameters using EM images. Bivariate correlation analysis was performed between change in PLIN4 mRNA expression levels and change in area of LDs in the SS (B) and IMF regions (C) after 12 weeks of training (n = 18).