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. 2015 Aug 11;3(8):e12481. doi: 10.14814/phy2.12481

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Intramuscular adipocytes. (A) Sections of paraffin-embedded biopsies from m. vastus lateralis (n = 8) were stained with PLIN1 antibody (red, mouse anti-PLIN1) to detect adipocytes (A). Representative images (200X) were obtained by wide-field fluorescence microscopy. High magnification (1000X) of adipocytes is shown in the insert. (B) The sections were stained for PLIN4 (green, rabbit anti-PLIN4) and PLIN1 (red, mouse anti-PLIN1), showing partly co-localization in intramuscular adipocytes (n = 4). The merged image (200X) shows co-localization of the proteins (white arrow). The image was obtained by confocal fluorescence microscopy.