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. 2015 Aug 26;3(8):e12482. doi: 10.14814/phy2.12482

Figure 6.

Figure 6

Cocaine induces fibronectin expression in L-LECs. (A) Cocaine-induced FN expression in L-LECs by confocal microscopy. L-LECs were cultured in chamber slides and incubated with cocaine or vehicle control (Ctrl) for 18 h before fixing and staining cells. Green = FN; Blue = DAPI. Scale bars = 20 μm. (B) The effect of cocaine on gp120-induced FN expression in L-LECs and conditioned media by western blot analysis. L-LECs were incubated with cocaine (50 μmol/L) or vehicle control (Ctrl) for 2 h before adding HIV-1 gp120 for another 18 h. FN expression was assessed in the conditioned media and total cell lysates (TCL). GAPDH used as loading control. Densitometry analysis was done using ImageJ software. The results are shown in (C) and (D).