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. 2015 Aug 26;3(8):e12482. doi: 10.14814/phy2.12482

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Sigma-1R is involved in cocaine-induced FN expression in L-LECs. (A) The effect of sigma-1R inhibition on cocaine-induced FN expression. L-LECs were serum-starved for 1 h, then pretreated with sigma-1R inhibitor (1 μmol/L) or 1 × PBS for 1 h before incubating with cocaine for 18 h, as indicated. GAPDH used as loading control. The experiment was repeated more than three times. (B) Densitometry analysis was done using ImageJ software. The result is shown. (C) siRNAs to D4R inhibit expression of D4R and of sigma-1R (σ-1R) in L-LECs. L-LECs were transfected with D4R-specific siRNAs (+) and with nontargeting siRNAs (−). Protein expression was assessed by western blot analysis 48 h later. GAPDH used as loading control. The experiment was repeated more than three times. The results of densitometry analysis are shown in (D).