Skip to main content
. 2015 Aug 11;3(8):e12485. doi: 10.14814/phy2.12485

Table 2.

The primers used in this study

Function classifications Gene name Sense and antisense primer (5′–3′) Size (bp) GenBank No.
Internal reference β-actin CAGGATGCAGAAGGAGATCACA 92 KJ126772
CGATCCAGACGGAGTATTTACG
Elongation factor 1 alpha (EF1α) CTACGTGACCATCATTGATGCC 106 KJ123689
AACACCAGCAGCAACGATCA
Regulatory factors Peroxisome proliferator-activated receptor alpha (PPARα) CTGATAAAGCTTCGGGCTTCCA 106 KF871430
CGCTCACACTTATCATACTCCAGCT
Peroxisome proliferator-activated receptor beta (PPARβ) CAGGAGAACAGTGACAACAAGCA 100 KF751705
CCAAACGCAGCTGTTCTGAGA
Peroxisome proliferator-activated receptor gamma variant 1 (PPARγ1) TCCAGCTTCAGAAACAGAGAGTGTG 111 KF918712
AGACTGAAGCCAACAGGCCA
Peroxisome proliferator-activated receptor gamma variant 2 (PPARγ2) CAGGCAGAGATTTTACCCATCAAAC 104 KF918713
TGCTGTGTTCAGACTGAAGCCAA
Sterol regulatory element-binding transcription factor 1 (SREBP1) TGCAGCAGAGAGACTGTATCCGA 102 XM_005457771
ACTGCCCTGAATGTGTTCAGACA
Lipid uptake Fatty acid transport protein 5 (FATP5) TACACATCTGGGACCACAGGTTTG 110 XM_003443859.2
AAGATGTCCTCTGCTGTGACTCCA
Cluster determinant 36 (CD36) TGGAGCACTGGACATCAGTTCCT 96 XM_003452029
CCCAACACAACCTCCCGTAGATAT
Hepatic lipase (HL) GCAGACGCTACAGGAGCACTACAA 97 FJ436083.1
AAAGCTCCCAGCAAATCCAGAGAT
Lipoprotein lipase (LPL) CACCAAACTAGTGGGTCGTGATGT 103 NM_001279753
TCCCAGACTATAACCCAGCAGATGA
Intracellular fatty acid transportation Fatty acid-binding protein 4 (FABP4) AAGCTGGGAGAGGAGTTTGATGAA 112 XM_003458335
TCTCTTTGCCGTCCCACTTCT
Fatty acid synthesis Acetyl-CoA carboxylase alpha (ACCα) TAGCTGAAGAGGAGGGTGCAAGA 110 XM_005471970
AACCTCTGGATTGGCTTGAACA
ATP citrate lyase (ACLY) AAAAGCTTTGATGAGCTTGGGG 102 XM_003442027
TACAGTGGGAGGAGGCAACTCTT
Fatty acid synthase (FAS) TCATCCAGCAGTTCACTGGCATT 102 GU433188
TGATTAGGTCCACGGCCACA
Glycerol synthesis Phosphoenolpyruvate carboxykinase 1 (PEPCK1) TGGAAGAACAAACCTTGGCG 99 XM_003448375
TGGGTCAATAATGGGACACTGTCT
TG synthesis Glycerol-3-phosphate acyltransferase 1 (GPAT) ATAACATCAAAGCCCCGCACAT 105 XM_005471309
CCATTCTTCGTCGTATGAAGAAACC
Diacylglycerol O-acyltransferase 2 (DGAT2) GCTTGAATTCTGTCACCCTGAAGA 106 XM_003458972
ACCTGCTTGTAGGCGTCGTTCT
Lipolysis Hormone-sensitive lipase (HSL) AACCTGGATGTCCATTTCTGGAAG 102 FJ601660
TCGGTTTACCTTGACTTGAGTGGA
Adipose triglyceride lipase (ATGL) AAAACGTCCTGGTGACCCAGT 104 XM_003440346
TAGGAGGAATGATGCCACAGTACA
Fatty acid β-oxidation Acetyl-CoA carboxylase beta (ACCβ) ACATGCAGTCCATGCTGCGT 106 XM_003451659
AAATGCCTCTCAAGCCACTCAA
Carnitine palmitoyltransferase I alpha (CPT1a) TTTCCAGGCCTCCTTACCCA 102 XM_003440552
TTGTACTGCTCATTGTCCAGCAGA
Carnitine palmitoyltransferase I beta (CPT1b) AAGGGACGTTACTTCAAGGTG 101 GQ395696
TCCGACTTGTCTGCCAAGAT
Very low-density lipoprotein protein ApoB TCCCCAGCTACACTGCACAGTT 102 XM_005461940
CATCGCCTCTTCCTGACATCATC
ApoE ATAAGCTGCAGAAGCGCCTCAATA 106 XM_003447249
TTCACTGTATCCAGGTTCTGGGAG