Table 2.
The primers used in this study
| Function classifications | Gene name | Sense and antisense primer (5′–3′) | Size (bp) | GenBank No. |
|---|---|---|---|---|
| Internal reference | β-actin | CAGGATGCAGAAGGAGATCACA | 92 | KJ126772 |
| CGATCCAGACGGAGTATTTACG | ||||
| Elongation factor 1 alpha (EF1α) | CTACGTGACCATCATTGATGCC | 106 | KJ123689 | |
| AACACCAGCAGCAACGATCA | ||||
| Regulatory factors | Peroxisome proliferator-activated receptor alpha (PPARα) | CTGATAAAGCTTCGGGCTTCCA | 106 | KF871430 |
| CGCTCACACTTATCATACTCCAGCT | ||||
| Peroxisome proliferator-activated receptor beta (PPARβ) | CAGGAGAACAGTGACAACAAGCA | 100 | KF751705 | |
| CCAAACGCAGCTGTTCTGAGA | ||||
| Peroxisome proliferator-activated receptor gamma variant 1 (PPARγ1) | TCCAGCTTCAGAAACAGAGAGTGTG | 111 | KF918712 | |
| AGACTGAAGCCAACAGGCCA | ||||
| Peroxisome proliferator-activated receptor gamma variant 2 (PPARγ2) | CAGGCAGAGATTTTACCCATCAAAC | 104 | KF918713 | |
| TGCTGTGTTCAGACTGAAGCCAA | ||||
| Sterol regulatory element-binding transcription factor 1 (SREBP1) | TGCAGCAGAGAGACTGTATCCGA | 102 | XM_005457771 | |
| ACTGCCCTGAATGTGTTCAGACA | ||||
| Lipid uptake | Fatty acid transport protein 5 (FATP5) | TACACATCTGGGACCACAGGTTTG | 110 | XM_003443859.2 |
| AAGATGTCCTCTGCTGTGACTCCA | ||||
| Cluster determinant 36 (CD36) | TGGAGCACTGGACATCAGTTCCT | 96 | XM_003452029 | |
| CCCAACACAACCTCCCGTAGATAT | ||||
| Hepatic lipase (HL) | GCAGACGCTACAGGAGCACTACAA | 97 | FJ436083.1 | |
| AAAGCTCCCAGCAAATCCAGAGAT | ||||
| Lipoprotein lipase (LPL) | CACCAAACTAGTGGGTCGTGATGT | 103 | NM_001279753 | |
| TCCCAGACTATAACCCAGCAGATGA | ||||
| Intracellular fatty acid transportation | Fatty acid-binding protein 4 (FABP4) | AAGCTGGGAGAGGAGTTTGATGAA | 112 | XM_003458335 |
| TCTCTTTGCCGTCCCACTTCT | ||||
| Fatty acid synthesis | Acetyl-CoA carboxylase alpha (ACCα) | TAGCTGAAGAGGAGGGTGCAAGA | 110 | XM_005471970 |
| AACCTCTGGATTGGCTTGAACA | ||||
| ATP citrate lyase (ACLY) | AAAAGCTTTGATGAGCTTGGGG | 102 | XM_003442027 | |
| TACAGTGGGAGGAGGCAACTCTT | ||||
| Fatty acid synthase (FAS) | TCATCCAGCAGTTCACTGGCATT | 102 | GU433188 | |
| TGATTAGGTCCACGGCCACA | ||||
| Glycerol synthesis | Phosphoenolpyruvate carboxykinase 1 (PEPCK1) | TGGAAGAACAAACCTTGGCG | 99 | XM_003448375 |
| TGGGTCAATAATGGGACACTGTCT | ||||
| TG synthesis | Glycerol-3-phosphate acyltransferase 1 (GPAT) | ATAACATCAAAGCCCCGCACAT | 105 | XM_005471309 |
| CCATTCTTCGTCGTATGAAGAAACC | ||||
| Diacylglycerol O-acyltransferase 2 (DGAT2) | GCTTGAATTCTGTCACCCTGAAGA | 106 | XM_003458972 | |
| ACCTGCTTGTAGGCGTCGTTCT | ||||
| Lipolysis | Hormone-sensitive lipase (HSL) | AACCTGGATGTCCATTTCTGGAAG | 102 | FJ601660 |
| TCGGTTTACCTTGACTTGAGTGGA | ||||
| Adipose triglyceride lipase (ATGL) | AAAACGTCCTGGTGACCCAGT | 104 | XM_003440346 | |
| TAGGAGGAATGATGCCACAGTACA | ||||
| Fatty acid β-oxidation | Acetyl-CoA carboxylase beta (ACCβ) | ACATGCAGTCCATGCTGCGT | 106 | XM_003451659 |
| AAATGCCTCTCAAGCCACTCAA | ||||
| Carnitine palmitoyltransferase I alpha (CPT1a) | TTTCCAGGCCTCCTTACCCA | 102 | XM_003440552 | |
| TTGTACTGCTCATTGTCCAGCAGA | ||||
| Carnitine palmitoyltransferase I beta (CPT1b) | AAGGGACGTTACTTCAAGGTG | 101 | GQ395696 | |
| TCCGACTTGTCTGCCAAGAT | ||||
| Very low-density lipoprotein protein | ApoB | TCCCCAGCTACACTGCACAGTT | 102 | XM_005461940 |
| CATCGCCTCTTCCTGACATCATC | ||||
| ApoE | ATAAGCTGCAGAAGCGCCTCAATA | 106 | XM_003447249 | |
| TTCACTGTATCCAGGTTCTGGGAG |