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. 2015 Aug 19;3(8):e12507. doi: 10.14814/phy2.12507

Figure 1.

Figure 1

Intraocular and intracranial pressure elevation methodology. (A) IOP and ICP elevation was achieved by placing a needle into the vitreous chamber (VC) and a dual-lumen needle into the ipsilateral lateral ventricle (LV), respectively. A saline reservoir was connected to the vitreous chamber needle, whereas a syringe pump was connected to the lateral ventricle cannula. (B) A custom made dual-lumen needle with infusion (inner needle) and measurement ports (outer needle) was used for ICP elevation. (C) Vitreous chamber cannulation employed a 27G needle inserted 2 mm behind the limbus at a 45 degree angle. (D) At each ICP level, IOP was elevated from 10 to 70 mmHg in 10 mmHg steps each lasting 3 min. Optical coherence tomography (OCT) or electroretinography (ERG) was assayed at each IOP and ICP level.