Fig 7. Arginine is sensed by RovM to control biofilm production.
(A) Biofilm formation of Y. pestis KIM6+ strains in TMH-gal+arg-. Error bars are the mean±SD of four independent biological replicates. A One Way ANOVA combined with a Tukey’s test was used to test significant differences in biofilm formation between strains. The overall P-value for the ANOVA was P<0.0001. Significant differences in biofilm formation are indicated by * for P<0.05 and *** for P<0.001. (B) Growth of Y. pestis strains in TMH-gal+arg-. Error bars are the mean±SD of four independent biological replicates. The mean mean±SD of the growth rate (μ) is given in parentheses. All closed symbol growth curves represent strains containing multiple copies of rovM. A One Way ANOVA combined with a Tukey’s test was used to test significant differences in the growth rate between strains. The overall P-value was P<0.0001. Significant difference in growth rates is indicated by *** for a P-value of 0.0001. Horizontal lines group a set of bars together. Capped lines mark the two bars (or groups of bars) that are being compared.