Fig 8. RT-qPCR of rovM and rovA in minimal medium containing arginine.
Quantitative transcript levels of (A) rovM and (B) rovA genes from exponential phase planktonic cultures of wild type (pACYC177), ΔrovM (pACYC177) mutant, wild type (pCRrovM) and ΔrovM (pACrovM) complemented mutant in Sebbane minimal medium supplemented with arginine. Error bars represent the mean±SD of at least 3 independent replicates. A One Way ANOVA combined with a Tukey’s post-test was used to test significant differences in transcript level. The overall P-value for the ANOVAs was 0.0003 for (A) and 0.0173 for (B). Significant differences in gene transcript levels between strains is indicated by *for P<0.05 and *** for P<0.001 Average fold increase in expression±SD of rovM and rovA is indicated in parentheses. WT = wild type, ns = not significant.