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. 2015 Sep 8;10(9):e0137138. doi: 10.1371/journal.pone.0137138

Fig 1. Characterization of glycosylated and unglycosylated Kv3.1a and Kv1.1 proteins heterologously expressed in B35 neuroblastoma cells.

Fig 1

Western blots of wild type (Wt) and N220Q/N229Q Kv3.1a proteins from total membranes (a), and also Wt Kv3.1a protein samples digested (+) and undigested (-) with PNGase F, Endo H, or neuraminidase (neu), as indicated (b). Immunoblots of Wt and N207Q Kv1.1 proteins from transfected B35 cells (c), and also Wt Kv1.1 protein samples digested (+) and undigested (-) with glycosidases, as shown (d). Vertical solid grey line indicates a separate blot. The numbers adjacent to the Western blots represent the Kaleidoscope markers. Black arrows and black solid lines denote glycosylated wild type Kv proteins while grey arrows and black dashed lines represent unglycosylated Kv proteins. Grey dashed line signifies glycosylated Kv3.1a with sialic acid removed. Dashed arrow denotes Kv3.1a with oligomannose type N-glycans.