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. 2015 Sep 8;10(9):e0137366. doi: 10.1371/journal.pone.0137366

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© 2015 Kim et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — PBMC migration was evaluated in 96 well Transwell-plates (pore size: 5 μm). Medium containing 10% FCS served as a positive control (PC), medium containing 0.5% FCS served as a negative control (NC). Supernatants of HAT and IAT were collected in medium containing 0.5% FCS. MIF and MCP-1-dependent effects were shown by use of monoclonal anti-MIF and anti-MCP-1 antibody and an IgG isotype control. The chemotactic index represents the ability of the sample to induce cell migration. Each sample was normalized to the negative control. Data are presented in mean chemotactic index ± SEM. Statistically significant differences are indicated by asterisks (*, p<0.05; **, p<0.01; ***, p<0.001). A PBMC migration towards supernatants of HAT and IAT compared to PC and NC. B PBMC migration toward IAT partially neutralized by MIF and MCP-1 antibodies.